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Dr. Zavos has been a true pioneer! He has had a long career as
a World renowned authority in human reproduction, and is the chief
scientist in the development of numerous new and innovative ideas
and technologies in the human reproductive area with worldwide
implications.
Below is a list of pioneering ideas and technologies developed
by Dr. Zavos in the field of reproductive medicine and assisted
reproductive technologies.
 Created
the First Human-Bovine Hybrid Cloned Embryo
Illmensee
K., Levanduski M., Zavos P.: Evaluation of the embryonic
preimplantation potential of human adult somatic cells
via an embryo interspecies bioassay using bovine oocytes.
Fertility and Sterility 85 (Suppl 1): 1248-1260, 2006.
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First to develop
Embryo Splitting technologies using mouse blastomere biopsy
for future use in human assisted reproductive technologies.
 Illmensee
K., Kaskar K., Zavos P.: Efficient
blastomere biopsy for mouse embryo splitting for future
applications in human ART. Reproductive Bio-Medicine
Online, 11 (6): 716–725, 2005.
Illmensee K., Kaskar K., Zavos P.:In-vitro blastocyst
development from serially split mouse embryos: future
implications for human ART. Fertil. Steril. 86 suppl.
4:1112-20, 2006.
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Created
the First Human-Bovine Hybrid Cloned Embryo from Post-Mortem
Tissues. |
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Attempted
the First Fresh Human Cloned Embryo Transfer for Reproductive
Purposes. |
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PRESS
RELEASE: FEBRUARY 2, 2004
MSNBC
- Doctor claims he implanted human clone
Zavos P, Illmensee K: Possible treatment
of male infertility by reproductive cloning: Technique
for creating cloned human four-cell embryo and subsequent
embryo transfer. Arch. Androl. 52:243-254, 2006. |
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Created
the first ever human cloned embryo for reproductive purposes.
 Human
Reproductive Cloning: The Time is Near (RBM Online, 2003)
A
Clone at Last? (US News and World Report, April 21, 2003)
First Proof
of Cloned Embryo (Sunday Herald, Glasgow, April 6, 2003)
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Participated in the establishment
of the first IVF program in Kentucky and the subsequent birth
of the first IVF baby in the State of Kentucky, USA, 1986.
TEST-TUBE
BABIES. -- June 18, 1986
TEST-TUBE BABIES. "1st 'test-tube' baby conceived in Kentucky is born" The
first "test-tube baby"
conceived in Kentucky was born yesterday to a Lexington couple
at the University of Kentucky Albert B. Chandler Medical
Center.
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Participated
in the development and introduction of the first Intra-Uterine
Insemination (IUI) procedures in humans in the World.
This
was done in collaboration with Dr. Melvin R. Cohen, the Director
of the Andrology Institute of Chicago, at Northwestern University.
The work was published during the International Symposium
on Human Artificial Insemination and Semen Preservation in
Paris, France, in 1979.
Zavos, P.M. and Cohen, M.R.: Mucus penetration
test as an assay for frozen and fresh spermatozoa. International
Symposium on Human Artificial Insemination and Semen
Preservation, Paris, France. p. 52. April 9-11, 1979.
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Employment of Test-Yolk buffer
for semen refrigeration using human semen
Zavos, P.M., Goodpasture, J.C., Zaneveld, L.J.D. and
Cohen, M.R.: Motility and enzyme activity of human spermatozoa
stored for 24 hours at +5 C and -196 C. Fertil. Steril.,
34(16):607-609, 1980.
Zavos, P.M., Correa, J.R., Foster, C.L.,
Massey, J.B., Zarmakoupis-Zavos, P.N.: Fertilization potential
and qualitative characteristics of human spermatozoa after
short-term cryostorage at 5C in two different TEST-Yolk
buffer preparations.Tohoku Journal of Experimental
Medicine, 184:1-10, 1998
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Employment of bovine
cervical mucus as a bioassay for fresh and cryopreserved
human spermatozoa.
Zavos, P.M. and Cohen, M.R.: Bovine mucus penetration
test: An assay for fresh and cryopreserved human spermatozoa.
Fertil. Steril., 34(2):175-176, 1980.
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Employment of supercooling
as a means to cryostore spermatozoa.
Zavos, P.M. and Graham, E.F. Supercooling effects on motility
and fertility of turkey spermatozoa as compared between
two different buffers. Cryo-Letters, 2:76-85, 1981.
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Employment of emulsions
and supercooling methods as a means to cryostore spermatozoa.
Zavos, P.M. and Graham, E.F. Preservation of turkey spermatozoa
by the use of emulsions and supercooling methods. Cryobiology,
18:497-505, 1981.
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Employment of "seeding" to
limit or prevent cryo-damage to cell suspensions during freezing.
Zavos, P.M. and Graham, E.F.: Effects of various degrees
of supercooling on motility and fertility of turkey spermatozoa.
Cryo-Letters, 3:71-82, 1982.
Zavos, P.M. and Graham, E.F.: Effects of various degrees
of supercooling and nucleation temperatures on fertility
of frozen turkey spermatozoa. Cryobiology, 20:553-559,
1983.
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Documentation of
the presence of opisthosmotic shock to frozen-thawed human
spermatozoa.
Zavos, P.M.: Opisthosmotic shock of frozen-thawed human
spermatozoa. Infertility, 5(3):247-255, 1983.
Zarmakoupis-Zavos, P.N., Correa, J.R., Aslanis, P., Antypas,
S., Zavos, P.M.: Occurrence of osmotic
shock in human spermatozoa: its effects on qualitative
measurements in frozen-thawed spermatozoa. Middle East
Fertility Society Journal, 3(1):66-71, 1998.
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Employment of a
non-invasive technique for harvesting and reconstituting
retrograde ejaculates.
Zavos, P.M. and Wilson, E. A.: Retrograde ejaculation:
A new technique for collection and reconstitution of retrograde
ejaculate. Infertility, 5(4):287-296, 1983.
Zavos, P.M. and Wilson, E.A.: Ejaculation retrograde:
etiologie et traitement. Emploi d'une nouvelle technique.
Contraception- fertilite-sexualite, 12(6):813-818, 1984.
Zavos, P.M. and Wilson, E.A.: Retrograde ejaculation:
etiology and treatment via the use of a new noninvasive
method. Fertil. Steril., 42(4):627-632, 1984.
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Employment of seminal
collection devices in order to enhance ejaculate characteristics
as compared to those ejaculates collected via masturbation.
Zavos, P.M.: Characteristics of human
ejaculates collected via masturbation and a new Silastic
seminal fluid collection device (SCD). Fertil. Steril.,
43(3):491-492, 1985.
Zavos, P.M.: Seminal parameters of ejaculates
collected from oligospermic and normospermic patients via
masturbation and at intercourse with the use of a Silastic
seminal fluid collection device. Fertil. Steril., 44(4):517-520,
1985.
Zavos, P.M.: Role de la stimulation sexuelle
dans l'elaboration de l'ejaculat chez l'homme. Contraception-fertilite-sexualite,
13(9):981-983, 1985.
Zavos, P.M.: Comparisons between ejaculates
collected at intercourse and at masturbation. Young Couples
International, 2:5, 1985.
Zavos, P.M.: Parameters and improvements
in ejaculates collected at intercourse and the use of a
silastic seminal collection device vs masturbation. Infertility,
9(1):57-64, 1986.
Karagounis, S.C., Papanikolaou, N.A. and Zavos,
P.M.: Semen parameters compared between smoking
and non-smoking men: smoking intensity and semen parameters.
Infertility, 8(4): 373-382, 1985.
Zavos, P.M.: Incidence des pertes de
sperme au cours de l'ejaculation provoquee' par masturbation.
Contraception-fertilite-sexualite, 14(11):1003-1006, 1986.
Zavos, P.M.: Treatment of ejaculatory
and spermatogenic dysfunctions in oligospermic patients
via collection of ejaculates at intercourse using a seminal
fluid collection device. Infertility, 10(2):167-171, 1987.
Zavos, P.M.: Utilisation clinique d'un
dispositif de recueil du sperme (SCD); parametres seminaux
et ameliorations d'ejaculats recueillis par rapport sexuel
avec le SCD et par masturbation, chez des patients presentant
divers dysfonctionnements spermatogenes et ejaculatoires.
Contraception-fertilite-sexualite, 16(6):475-481, 1988.
Zavos, P.M., Goodpasture, J.C.: Clinical
use of a seminal collection device: improvements of specific
seminal deficiencies through use of a seminal collection
device at intercourse versus masturbation. Infertility,
11(4):289-304, 1988.
Zavos, P.M. and Goodpasture, J.C.: Clinical
improvements of specific seminal deficiencies via intercourse
with a seminal collection device versus masturbation. Fertil.
Steril. 51(1):190-193, 1989.
Zarmakoupis-Zavos, P.N., Zarmakoupis, C.N., Correa, J.R., Zavos,
P.M.: Multiple ejaculate collection via the
use of a semen collection device at intercourse versus
masturbation. Middle East Fertility Society
Journal, 4 (3): 228-232, 1999.
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Documentation of
the effects of smoking on seminal characteristics and sperm
parameters in humans.
Zavos, P.M.: Cigarette smoking and human reproduction:
Effects on female and male fecundity. Infertility 12(1):35-46,
1989.
Zavos, P.M.: Tabagisme: sterilite masculine et femenine.
Contraception-fertilite-sexualite, 17(2):133-138, 1989.
Zavos, P.M., Correa, J.R., Antypas, S.,
Zarmakoupis-Zavos, P.N., Zarmakoupis C.N.: The effects
of seminal plasma from cigarette smokers on sperm viability
and longevity. Fertility and Sterility, 69(3):425-429,
1998.
Zavos, P.M., Correa, J.R., Karagounis,
C.S., Ahparaki, A., Phoroglou, C.R., Hicks, C.H., Zarmakoupis-Zavos,
P.N.: An electron microscope study of the axonemal ultrastructure
in human spermatozoa from smoking and nonsmoking males.
Fertility and Sterility, 69(3):430-434, 1998.
Zavos, P.M.: Cigarette smoking effects
on human reproduction. Journal of the Franklin Institute,
335(A):1-8, 1998.
Zavos, P.M., Zarmakoupis, C.N. and Zarmakoupis-Zavos,
P.N.:The impact of cigarette smoking on human reproduction:
its effects on female and male fecundity. Middle East Fertility
Society Journal, 3(3):340-47, 1998.
Zavos, P.M.: Impact of cigarette smoking
on human reproduction: Its effects on male and female fecundity.
Technology, 6:9-16, l999.
Zavos, P.M. and Zarmakoupis-Zavos, P.N.:
How smoking affects reproductive health: Female fecundity.
OBG Management, 2:48-55, l999.
Zavos, P.M.: Cigarette smoking and human reproduction:
effects on female and male fertility. American Council
on Science and Health (Special Report) 12(3):16-19,2000.
Karagounis, C.S., Papanikolaou, N.A. and Zavos,
P.M.: Cigarette smoking: Smoking intensity and semen parameters
in men. Proceedings of the Fifth World Congress on Human
Reproduction, Supplement, pp.135-137, 1986.
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Introduction of the first commercial
and patented semen preparation device for preparation of
human sperm for IUI purposes.
Zavos, P.M. and Centola, G.M.: Qualitative and quantitative
improvements in human spermatozoa recovered via the swim-up
and a new semen filtration column (SFC) method. Infertility,
13(1):25-34, 1990.
Zavos, P.M. and Centola, G.M.: Ameliorations seminales
des spermatozoides utilises pour la fecondation artificielle:
Comparaison entre la methode de "nage ascedante" et
une nouvelle methode de filtration du sperme sur colonne.
Contraception-fertilite-sexualite, 18(11):943-948, 1990.
Zavos, P.M.: Selection de spermatozoides viables a partit
d'echantillons de spermes humains congeles-decongeles:
comparaison de la methode du "swim-up" et d'une
nouvelle methode de filtration : le SpermPrepT. Contraception-fertilite-sexualite,
19(4):293-297, 1991.
Zavos, P.M., Sofikitis, N., Toda, T. and Miyagawa, I.:
Improvements in qualitative characteristics of cryopreserved
human spermatozoa following recovery via the SpermPrepTII
filtration method. Tohoku J. Experimental Medicine. 163:283-290,
1991.
Zavos, P.M.: Preparation of human frozen-thawed specimens
using the SpermPrepT filtration method: Improvements over
the conventional swim-up method. Fertility and Sterility.
57(6):1326-1330, 1992.
Zavos, P.M.: Amelioration des caracteristiques qualitatives
des spermatozoides humains cryoconserves apres recuperation
par la methode de filtration SpermPrepTII. Contraception-fertilite-sexualite.
20(5):541-545, 1992.
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Employment of the hypoosmotic
swelling test (HOS) to assess functional integrity of equine
sperm membrane.
Zavos, P.M.: The use of hypoosmotic swelling test (HOS)
to assess functional integrity of equine sperm membrane.
J. Assisted Reproductive Technology-Andrology. 2(2):215-216,
1991.
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Introduction of
the first standardized and patented technology for in-office
use for semen preparation for IUI purposes.
Zavos, P.M.: Improvements in qualitative and quantitative
characteristics of human spermatozoa using the Swim-up
method and a new standardized Rise-KitT technique. Molecular
Andrology. 4:231-239, 1992.
Zavos, P.M., Zarmakoupis-Zavos, P.N.
Correa, J.R., Aslanis, P., Zarmakoupis, C.N.: Assessment
of two devices for in vitro preparation of human sperm.
Archives of Andrology, 45(2):85-90, 2000.
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Employment of round
spermatid injections for treatment of patients with non-obstructive
azoospermia.
Sofikitis, N., Toda, T., Miyakawa, I., Zavos,
P.M., Pasyianos,
P., Mastelu, E.: Beneficial effects of electrical stimulation
before round spermatid nuclei injections into rabbit oocytes
on fertilization and subsequent embryonic development.
Fertility Sterility. 65(1):176-185, 1996.
Zavos, P.M., Barnes, F.L., Correa, J.R., Zarmakoupis-Zavos,
P.N., Tesarik, J.: Methods for isolation and purification
of post-ejaculate human round spermatids for possible use
in intracytoplasmic round spermatid injection. Middle East
Fertility Society Journal. 2:147-150, 1997.
Zavos, P.M.: Methods for isolation and
purification of post-ejaculate human round spermatids for
possible use in intracytoplasmic round spermatid injection.
Proceedings of the 5th International Congress
on Advances in Assisted Reproduction Technologies in the
Year 2000. Rome, Italy, p. 12-12/1, 1997.
Zavos, P.M., Antypas, S., Aslanis, P.,
Zarmakoupis, C.N and. Zarmakoupis-Zavos, P.N.: Development
of hyperosmotic shrinkage test to measure the functional
integrity of the membrane of human round spermatids retrieved
from testes for use in ICSI. Middle East Fertility Society
Journal, 5 (2), 115-119, 2000.
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Use of nonoxynol-9 co-precipitated
formulations with polyvinylpyrrolidone in rabbits as possible
vaginal contraceptives in humans.
Zavos, P.M., Correa, J.R.: Vaginal delivery
of new formulations of nonoxynol-9 co-precipitated with
polyvinylpyrrolidone in rabbits: comparisons between two
formulation-delivery systems. Contraception. 56(2):123-127,
1997.
Zavos, P.M., Correa, J.R., Zarmakoupis-Zavos,
P.N.: Assessment of a tablet drug delivery system incorporating
nonoxynol-9 co-precipitated with polyvinylpyrrolidone in
preventing the onset of pregnancy in rabbits. Fertility
and Sterility, 69(4):768-73, 1998.
Digenis, G.A., Nosek, D., Mohammadi., F., Darwzeh, N.B.,
Anwar, H.S. and Zavos, P.M.: Novel vaginal
controlled delivery systems incorporating coprecipitates
of nonoxynol-9. Pharmaceutical Development and Technology,
4(3):421-430, 1999.
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Introduction of the Viscolytic
System (VLS), a commercial product for treatment of seminal
viscosity difficulties.
Zavos, P.M., Correa, J.R., Zarmakoupis-Zavos,
P.N.: Effect of treatment of seminal viscosity difficulties
with -chymotrypsin on the recovery of spermatozoa for assisted
reproductive technologies: comparisons between the SpermPrepT
filtration and Percoll gradient centrifugation methods.
Middle East Fertility Society Journal,2 (3):223-29,
1997.
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Employment of Test Yolk
buffer (TYB) as a means to treat antisperm antibodies in
humans
Zavos, P.M., Correa, J.R., Zarmakoupis-Zavos,
P.N.: Antisperm antibody treatment mode: levels of antisperm
antibodies following incubation in TEST-Yolk buffer and
filtration via the SpermPrepTII method. Fertility and Sterility,
69(3):517-21, 1998.
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Introduction of the first
commercial liquid semen shipper for transporting unfrozen
semen to a location for evaluation purposes or for use
in an insemination program or for other Assisted Reproductive
Techniques (ART) such as In-Vitro Fertilization (IVF).
Zavos, P.M., Correa, J.R., Clark, W.,
Zarmakoupis-Zavos, P.N.:Sperm viability in human semen
specimens cryostored at 5°C using the Bio-TranzT container
system for semen transport. Middle East Fertility Society
Journal, 3(3): 325-329, 1998.
Zavos, P.M., Kaskar, K., Zarmakoupis-Zavos,
P.N.: Evaluation of human semen specimens transported at
5°C using the Bio-TranzT shipping system. The 25th Annual
Meeting of the American Society of Andrology, Boston, MA,
USA; April 7-11, 2000.
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Introduction of the first
standardized and patented multi-chamber technology for
the recovery of high quality spermatozoa for IUI or other
forms of assisted reproductive technologies.
Zavos, P.M., Abou Abdallah, M., Aslanis,
P., Correa, J.R., Zarmakoupis-Zavos, P.N.: Use of the Multi-ZSCT
One-Step Standardized Swim-Up Method: Recovery of High
Quality Spermatozoa for IUI or Other Forms of Assisted
Reproductive Technologies. Fertil. Steril. 74 (4), 834-835,
2000.
Correa-Pérez, J.R., Torres-Santiago, O, Fernández-Pelegrina,
R., Zavos , P.M.: Sperm characteristics
recovered from teratozoospermic specimens using a multi-layer
swim-up column. The 25th Annual Meeting of the
American Society of Andrology, Boston, MA, USA; April 7-11,
2000.
Zavos, P.M., Kaskar, K., Zarmakoupis-Zavos,
P.N.: Evaluation of the Multi-ZSC column in selecting high
quality sperm from normozoospermic samples. The 16th Annual
Meeting of the European Society of Human Reproduction and
Embryology, Bologna, Italy, June 25-28, 2000.
Correa-Pérez, J.R., Torres-Santiago, O., Fernández-Pelegrina,
R., Zarmakoupis-Zavos, P.N., Zavos, P.M.:
Sperm quantitative/qualitative characteristics distribution
of normozoospermic and teratozoospermic specimens in a
multi-layer swim-up column. The 56th Annual
Meeting of the American Society for Reproductive Medicine,
San Diego, CA, USA, October 21-25, 2000.
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Introduced for the first
time the aging effect in human males
Zavos, P.M., Kaskar, K., Correa, J.R.,
Sikka, S.C., Zarmakoupis-Zavos, P.N.: Seminal characteristics
of men of different age groups: Is there an aging effect?
The 56th Annual Meeting of the American Society
for Reproductive Medicine, San Diego, CA, USA, October
21-25, 2000. (Requested for Press Release by ASRM)
Zavos, P.M., Aslanis, P., Kaskar, K., Correa, J.R., Zarmakoupis-Zavos,
P.N.: The aging effect of men on sexual behavior and seminal
characteristics: Fact or fiction? The 4th Panhellenic Andrology
Meeting, Athens, Greece, November 10-12, 2000. (Received
the Outstanding Clinical Research Award)
Zavos P.M., Kaskar K., Correa J.R., Sikka S.,
Zarmakoupis-Zavos P.N.: Seminal characteristics of men
of different age groups: Is there an aging effect? The
Asian Journal of Andrology, 2006 (Accepted, in press).
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Introduced for the first
time the impact of smoking has on the couples ability to
reproduce and the impact of cigarette smoking on male and
female sexual behavior.
Zavos, P.M., Zarmakoupis, C.N., Correa,
J.R., Zarmakoupis-Zavos, P.N.: The impact of cigarette
smoking on male and female sexual behavior: comparisons
between smoking and non-smoking couples. The 56th Annual
Meeting of the American Society for Reproductive Medicine,
San Diego, CA, USA, October 21-25, 2000.
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